{"id":77638,"date":"2019-02-04T13:18:20","date_gmt":"2019-02-04T12:18:20","guid":{"rendered":"https:\/\/cri1149.mlcom-dev.net\/flow-cytometry\/"},"modified":"2025-12-05T13:12:35","modified_gmt":"2025-12-05T12:12:35","slug":"flow-cytometry","status":"publish","type":"page","link":"https:\/\/cri1149.mlcom-dev.net\/en\/platforms\/flow-cytometry\/","title":{"rendered":"Flow cytometry"},"content":{"rendered":"<p style=\"text-align: justify;\">The CRI flow cytometry platform is open to anyone wishing to use the equipment offered by the infrastructure. The platform offers the possibility of training in the techniques of acquisition, processing and analysis of samples.<\/p>\n<p style=\"text-align: justify;\">It provides self-service, four analyzers and a sorter but also offers ad hoc assistance in projects requiring mastery of this technology.<\/p>\n<p style=\"text-align: justify;\">Image cytometry is only offered as part of a service (data acquisition by an engineer)<\/p>\n<p style=\"text-align: justify;\">The Charter must be completed, signed and submitted to the platform managers for validation before any use.<\/p>\n<h4><b> Services offered <\/b><\/h4>\n<p>&#8211; <strong>Analyses<\/strong><\/p>\n<ol>\n<li>Multi membrane and intracellular markings<\/li>\n<li>Signaling lanes<\/li>\n<li>Cell cycle<\/li>\n<li>Apoptosis<\/li>\n<li>Molecular analysis in multiplex<\/li>\n<li>Analysis of transfected cells ..<\/li>\n<\/ol>\n<p>&#8211; <strong> Image cytometry (Imagestream MKII) <\/strong><\/p>\n<p>&#8211; <strong> Training<\/strong><\/p>\n<p>&#8211; <strong> Help in setting up or improving an experimental protocol <\/strong><\/p>\n<p>&#8211; <strong> Help in the analysis and interpretation of results <\/strong><\/p>\n<p>&nbsp;<\/p>\n<h4><b> Our equipment<\/b><\/h4>\n<ul>\n<li>\n<h5><strong><span style=\"text-decoration: underline;\">Spectral cytometer<\/span><\/strong><\/h5>\n<\/li>\n<\/ul>\n<p><b>&#8211; Sony ID7000 <\/b><b>: 6 lasers<br \/>\n<\/b><em>320 nm, 355 nm, 405 nm, 488 nm, 561 nm et 633 nm<\/em><\/p>\n<ul>\n<li>\n<h5><strong><span style=\"text-decoration: underline;\">Flow cytometer:<\/span><\/strong><\/h5>\n<\/li>\n<\/ul>\n<p><b> &#8211; BD <\/b><b>Fortessa<\/b> <b> X20: 22 parameters (5 lasers) <br \/>\n<\/b> <em> Violet (405nm), Blue (488nm), Green-yellow (561nm), Red (638nm), UV (365nm) <\/em><\/p>\n<p><em><strong>&#8211;<\/strong><\/em> <strong> BD Fortessa: 18 parameters (4 lasers) <\/strong> <em> <br \/>\nViolet (405 nm), Blue (488 nm), Green-yellow (561 nm), Red (638 nm) <\/em><\/p>\n<p><strong> &#8211; BD Canto II: 10 parameters (3 lasers) <\/strong> <br \/>\n<em> Violet (405 nm), Blue (488 nm), Red (633 nm) <\/em><\/p>\n<p><b> &#8211; Guava <\/b> <b>Easycyte<\/b> <b>: 6 parameters (2 lasers) <br \/>\n<\/b> <em> Blue (488 nm), Red (633 nm) <\/em><\/p>\n<p><strong> &#8211; Imagestream MKII (<b> (5 lasers) <br \/>\n<\/b><\/strong> <em> Violet (405nm), Blue (488nm), Green-yellow (561nm), Red (642nm), UV (375nm) <\/em> <strong> <br \/>\n<\/strong> <em> X20, X40, X60<\/em><\/p>\n<ul>\n<li>\n<h5><strong><span style=\"text-decoration: underline;\">Sorter:<\/span><\/strong><\/h5>\n<\/li>\n<\/ul>\n<p><strong>&#8211; Melody (3 lasers) <br \/>\n<\/strong> <em> Blue (488 nm) <\/em> <strong>, <\/strong> <em> Green-yellow (561 nm) <\/em> <strong>, <\/strong> <em> Red (633 nm) <br \/>\n2-way sorting, cloning module<\/em><\/p>\n<p>&nbsp;<\/p>\n<p><strong> <em> Access possible on request <\/em> <\/strong><\/p>\n<p>&#8211; BD Aria III Sorter: 18 parameters (4 lasers) <b> <br \/>\n<\/b> <em> Violet (405 nm), Blue (488 nm), Green-yellow (561 nm), Red (633 nm) <\/em> on request<\/p>\n<h5>Come to the platform<\/h5>\n<p>Center for Research on Inflammation <br \/>\nBichat site &#8211; 4th floor, room 456<\/p>\n<p>&nbsp;<\/p>\n<p style=\"text-align: center;\"><a href=\"https:\/\/grr.catibiomed.fr\/day.php?year=2023&amp;month=7&amp;day=3&amp;area=4\"><strong><span style=\"font-size: 18pt;\"> Reserve<\/span><\/strong><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>The CRI flow cytometry platform is open to anyone wishing to use the equipment offered by the infrastructure. The platform offers the possibility of training in the techniques of acquisition, processing and analysis of samples. It provides self-service, four analyzers and a sorter but also offers ad hoc assistance in projects requiring mastery of this [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":78639,"parent":77462,"menu_order":3,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-77638","page","type-page","status-publish","has-post-thumbnail","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Flow cytometry - CRI<\/title>\n<meta name=\"robots\" content=\"noindex, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Flow cytometry - CRI\" \/>\n<meta property=\"og:description\" content=\"The CRI flow cytometry platform is open to anyone wishing to use the equipment offered by the infrastructure. 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