{"id":77691,"date":"2020-07-24T11:26:37","date_gmt":"2020-07-24T09:26:37","guid":{"rendered":"https:\/\/cri1149.mlcom-dev.net\/technical-platform-of-ussing-chambers\/"},"modified":"2020-07-24T11:26:37","modified_gmt":"2020-07-24T09:26:37","slug":"technical-platform-of-ussing-chambers","status":"publish","type":"page","link":"https:\/\/cri1149.mlcom-dev.net\/en\/platforms\/technicals-platforms\/technical-platform-of-ussing-chambers\/","title":{"rendered":"Technical Platform of Ussing Chambers"},"content":{"rendered":"

The Ussing chambers, coupled to a voltage clamp allow to measure ex-vivo<\/em> the electrical characteristics of an epithelium and thus to measure the activity of the electrogenic transporters or parameters reflecting the permeability.<\/p>\n

The technical platform offers technical training for mounting the tissues in Chambers, and help for defining experimental design to answer specific questions and analyzing the results with appropriate softwares.<\/p>\n

Analyzes:<\/p>\n

    \n
  1. Measurement of electrogenic transporter activity<\/li>\n
  2. Measurement of parameters reflecting permeability (barrier function of an epithelium)\n
      \n
    • Measurement of transepithelial conductance (or resistance)<\/li>\n
    • Transfer of tracer molecules (FITC dextran-4Kd, FSA, HRP\u2026)<\/li>\n<\/ul>\n<\/li>\n
    • Measurement of molecule transfer\n
        \n
      • Xenobiotics<\/li>\n
      • Drugs<\/li>\n<\/ul>\n<\/li>\n<\/ol>\n

        Murine or human tissues studied:<\/p>\n

          \n
        • Intestinal, pulmonary, renal epith\u00e9lium\u2026<\/li>\n
        • Human biopsies<\/li>\n
        • Monolayer cells cultured in transwell (Caco-2, HT-29, MDCK\u2026)<\/li>\n<\/ul>\n

          Equipement:<\/p>\n

            \n
          • 3 Voltage clamp (DVC 1000 – World Precision Instruments)<\/li>\n
          • LabChart 8 acquisition and analysis software (ADInstruments)<\/li>\n
          • 6 Ussing chambers (Physiologic Instruments)<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"

            The Ussing chambers, coupled to a voltage clamp allow to measure ex-vivo the electrical characteristics of an epithelium and thus to measure the activity of the electrogenic transporters or parameters reflecting the permeability. The technical platform offers technical training for mounting the tissues in Chambers, and help for defining experimental design to answer specific questions […]<\/p>\n","protected":false},"author":9,"featured_media":79304,"parent":77644,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-77691","page","type-page","status-publish","has-post-thumbnail","hentry"],"acf":[],"yoast_head":"\nTechnical Platform of Ussing Chambers - CRI<\/title>\n<meta name=\"robots\" content=\"noindex, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Technical Platform of Ussing Chambers - CRI\" \/>\n<meta property=\"og:description\" content=\"The Ussing chambers, coupled to a voltage clamp allow to measure ex-vivo the electrical characteristics of an epithelium and thus to measure the activity of the electrogenic transporters or parameters reflecting the permeability. The technical platform offers technical training for mounting the tissues in Chambers, and help for defining experimental design to answer specific questions […]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/cri1149.mlcom-dev.net\/technical-platform-of-ussing-chambers\/?lang=en\" \/>\n<meta property=\"og:site_name\" content=\"CRI\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/CRI-Centre-de-recherche-sur-linflammation-100116118884153\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/cri1149.mlcom-dev.net\/technical-platform-of-ussing-chambers\/?lang=en\",\"url\":\"https:\/\/cri1149.mlcom-dev.net\/technical-platform-of-ussing-chambers\/?lang=en\",\"name\":\"Technical Platform of Ussing Chambers - CRI\",\"isPartOf\":{\"@id\":\"https:\/\/cri1149.mlcom-dev.net\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/cri1149.mlcom-dev.net\/technical-platform-of-ussing-chambers\/?lang=en#primaryimage\"},\"image\":{\"@id\":\"https:\/\/cri1149.mlcom-dev.net\/technical-platform-of-ussing-chambers\/?lang=en#primaryimage\"},\"thumbnailUrl\":\"https:\/\/cri1149.mlcom-dev.net\/wp-content\/uploads\/2023\/10\/ussing.png\",\"datePublished\":\"2020-07-24T09:26:37+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/cri1149.mlcom-dev.net\/technical-platform-of-ussing-chambers\/?lang=en#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/cri1149.mlcom-dev.net\/technical-platform-of-ussing-chambers\/?lang=en\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/cri1149.mlcom-dev.net\/technical-platform-of-ussing-chambers\/?lang=en#primaryimage\",\"url\":\"https:\/\/cri1149.mlcom-dev.net\/wp-content\/uploads\/2023\/10\/ussing.png\",\"contentUrl\":\"https:\/\/cri1149.mlcom-dev.net\/wp-content\/uploads\/2023\/10\/ussing.png\",\"width\":172,\"height\":208},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/cri1149.mlcom-dev.net\/technical-platform-of-ussing-chambers\/?lang=en#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Accueil\",\"item\":\"https:\/\/cri1149.mlcom-dev.net\/en\/cri-home\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Technical Platform of Ussing Chambers\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/cri1149.mlcom-dev.net\/#website\",\"url\":\"https:\/\/cri1149.mlcom-dev.net\/\",\"name\":\"CRI - Centre de Recherche sur l'Inflammation\",\"description\":\"Centre de Recherche sur l'Inflammation\",\"publisher\":{\"@id\":\"https:\/\/cri1149.mlcom-dev.net\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/cri1149.mlcom-dev.net\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"},{\"@type\":\"Organization\",\"@id\":\"https:\/\/cri1149.mlcom-dev.net\/#organization\",\"name\":\"CRI1149\",\"url\":\"https:\/\/cri1149.mlcom-dev.net\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/cri1149.mlcom-dev.net\/#\/schema\/logo\/image\/\",\"url\":\"https:\/\/cri1149.mlcom-dev.net\/wp-content\/uploads\/2014\/11\/8.4.2014-logo-CRI.jpg\",\"contentUrl\":\"https:\/\/cri1149.mlcom-dev.net\/wp-content\/uploads\/2014\/11\/8.4.2014-logo-CRI.jpg\",\"width\":215,\"height\":176,\"caption\":\"CRI1149\"},\"image\":{\"@id\":\"https:\/\/cri1149.mlcom-dev.net\/#\/schema\/logo\/image\/\"},\"sameAs\":[\"https:\/\/www.facebook.com\/CRI-Centre-de-recherche-sur-linflammation-100116118884153\",\"https:\/\/www.linkedin.com\/company\/cri-centre-de-recherche-sur-l-inflammation\/\"]}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Technical Platform of Ussing Chambers - CRI","robots":{"index":"noindex","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"og_locale":"en_US","og_type":"article","og_title":"Technical Platform of Ussing Chambers - CRI","og_description":"The Ussing chambers, coupled to a voltage clamp allow to measure ex-vivo the electrical characteristics of an epithelium and thus to measure the activity of the electrogenic transporters or parameters reflecting the permeability. The technical platform offers technical training for mounting the tissues in Chambers, and help for defining experimental design to answer specific questions […]","og_url":"https:\/\/cri1149.mlcom-dev.net\/technical-platform-of-ussing-chambers\/?lang=en","og_site_name":"CRI","article_publisher":"https:\/\/www.facebook.com\/CRI-Centre-de-recherche-sur-linflammation-100116118884153","twitter_card":"summary_large_image","twitter_misc":{"Est. reading time":"1 minute"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/cri1149.mlcom-dev.net\/technical-platform-of-ussing-chambers\/?lang=en","url":"https:\/\/cri1149.mlcom-dev.net\/technical-platform-of-ussing-chambers\/?lang=en","name":"Technical Platform of Ussing Chambers - CRI","isPartOf":{"@id":"https:\/\/cri1149.mlcom-dev.net\/#website"},"primaryImageOfPage":{"@id":"https:\/\/cri1149.mlcom-dev.net\/technical-platform-of-ussing-chambers\/?lang=en#primaryimage"},"image":{"@id":"https:\/\/cri1149.mlcom-dev.net\/technical-platform-of-ussing-chambers\/?lang=en#primaryimage"},"thumbnailUrl":"https:\/\/cri1149.mlcom-dev.net\/wp-content\/uploads\/2023\/10\/ussing.png","datePublished":"2020-07-24T09:26:37+00:00","breadcrumb":{"@id":"https:\/\/cri1149.mlcom-dev.net\/technical-platform-of-ussing-chambers\/?lang=en#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/cri1149.mlcom-dev.net\/technical-platform-of-ussing-chambers\/?lang=en"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/cri1149.mlcom-dev.net\/technical-platform-of-ussing-chambers\/?lang=en#primaryimage","url":"https:\/\/cri1149.mlcom-dev.net\/wp-content\/uploads\/2023\/10\/ussing.png","contentUrl":"https:\/\/cri1149.mlcom-dev.net\/wp-content\/uploads\/2023\/10\/ussing.png","width":172,"height":208},{"@type":"BreadcrumbList","@id":"https:\/\/cri1149.mlcom-dev.net\/technical-platform-of-ussing-chambers\/?lang=en#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Accueil","item":"https:\/\/cri1149.mlcom-dev.net\/en\/cri-home\/"},{"@type":"ListItem","position":2,"name":"Technical Platform of Ussing Chambers"}]},{"@type":"WebSite","@id":"https:\/\/cri1149.mlcom-dev.net\/#website","url":"https:\/\/cri1149.mlcom-dev.net\/","name":"CRI - Centre de Recherche sur l'Inflammation","description":"Centre de Recherche sur l'Inflammation","publisher":{"@id":"https:\/\/cri1149.mlcom-dev.net\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/cri1149.mlcom-dev.net\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Organization","@id":"https:\/\/cri1149.mlcom-dev.net\/#organization","name":"CRI1149","url":"https:\/\/cri1149.mlcom-dev.net\/","logo":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/cri1149.mlcom-dev.net\/#\/schema\/logo\/image\/","url":"https:\/\/cri1149.mlcom-dev.net\/wp-content\/uploads\/2014\/11\/8.4.2014-logo-CRI.jpg","contentUrl":"https:\/\/cri1149.mlcom-dev.net\/wp-content\/uploads\/2014\/11\/8.4.2014-logo-CRI.jpg","width":215,"height":176,"caption":"CRI1149"},"image":{"@id":"https:\/\/cri1149.mlcom-dev.net\/#\/schema\/logo\/image\/"},"sameAs":["https:\/\/www.facebook.com\/CRI-Centre-de-recherche-sur-linflammation-100116118884153","https:\/\/www.linkedin.com\/company\/cri-centre-de-recherche-sur-l-inflammation\/"]}]}},"post_type":"page","author_name":"Julien Tourneur-Marsille","_links":{"self":[{"href":"https:\/\/cri1149.mlcom-dev.net\/en\/wp-json\/wp\/v2\/pages\/77691","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/cri1149.mlcom-dev.net\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/cri1149.mlcom-dev.net\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/cri1149.mlcom-dev.net\/en\/wp-json\/wp\/v2\/users\/9"}],"replies":[{"embeddable":true,"href":"https:\/\/cri1149.mlcom-dev.net\/en\/wp-json\/wp\/v2\/comments?post=77691"}],"version-history":[{"count":0,"href":"https:\/\/cri1149.mlcom-dev.net\/en\/wp-json\/wp\/v2\/pages\/77691\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/cri1149.mlcom-dev.net\/en\/wp-json\/wp\/v2\/pages\/77644"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/cri1149.mlcom-dev.net\/en\/wp-json\/wp\/v2\/media\/79304"}],"wp:attachment":[{"href":"https:\/\/cri1149.mlcom-dev.net\/en\/wp-json\/wp\/v2\/media?parent=77691"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}