{"id":64205,"date":"2023-01-26T12:19:43","date_gmt":"2023-01-26T11:19:43","guid":{"rendered":"https:\/\/cri1149.mlcom-dev.net\/?post_type=publications&#038;p=64205"},"modified":"2025-09-11T16:47:58","modified_gmt":"2025-09-11T14:47:58","slug":"engineered-niches-support-the-development-of-human-dendritic-cells-in-humanized-mice","status":"publish","type":"publication","link":"https:\/\/cri1149.mlcom-dev.net\/en\/publication\/engineered-niches-support-the-development-of-human-dendritic-cells-in-humanized-mice\/","title":{"rendered":"Engineered niches support the development of human dendritic cells in humanized mice"},"content":{"rendered":"","protected":false},"featured_media":0,"template":"","meta":{"_acf_changed":false},"class_list":["post-64205","publication","type-publication","status-publish","hentry"],"acf":{"numero_de_publication":"Nat Commun.","date_de_publication":"20200428","numero_doi":"","equipe":[],"auteurs-liste":[{"texte_libre":true,"auteur-lien":null,"auteur-text":"Giorgio Anselmi"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Kristine Vaivode"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Charles-Antoine Dutertre"},{"texte_libre":false,"auteur-lien":16166,"auteur-text":""},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Yoann Missolo-Koussou"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Evan Newell"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Oliver Hickman"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Kristie Wood"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Alka Saxena"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Julie Helft"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Florent Ginhoux"},{"texte_libre":false,"auteur-lien":13178,"auteur-text":""}],"auteurs-manuel":"","liens_externes":null,"liens":[{"lien":"https:\/\/pubmed.ncbi.nlm.nih.gov\/32345968\/"}],"paragraphe":"<p>[:fr]<\/p>\r\n<h2 class=\"title\" style=\"text-align: justify;\">Abstract<\/h2>\r\n<div id=\"eng-abstract\" class=\"abstract-content selected\">\r\n<p style=\"text-align: justify;\">Classical dendritic cells (cDCs) are rare sentinel cells specialized in the regulation of adaptive immunity. Modeling cDC development is crucial to study cDCs and harness their therapeutic potential. Here we address whether cDCs could differentiate in response to trophic cues delivered by mesenchymal components of the hematopoietic niche. We find that mesenchymal stromal cells engineered to express membrane-bound FLT3L and stem cell factor (SCF) together with CXCL12 induce the specification of human cDCs from CD34<sup>+<\/sup>\u00a0hematopoietic stem and progenitor cells (HSPCs). Engraftment of engineered mesenchymal stromal cells (eMSCs) together with CD34<sup>+<\/sup>\u00a0HSPCs creates an in vivo synthetic niche in the dermis of immunodeficient mice driving the differentiation of cDCs and CD123<sup>+<\/sup>AXL<sup>+<\/sup>CD327<sup>+<\/sup>\u00a0pre\/AS-DCs. cDC2s generated in vivo display higher levels of resemblance with human blood cDCs unattained by in vitro-generated subsets. Altogether, eMSCs provide a unique platform recapitulating the full spectrum of cDC subsets enabling their functional characterization in vivo.<\/p>\r\n<\/div>\r\n<p>[:]<\/p>","paragraphe_en":"<p>[:fr]<\/p>\r\n<h2 class=\"title\" style=\"text-align: justify;\">Abstract<\/h2>\r\n<div id=\"eng-abstract\" class=\"abstract-content selected\">\r\n<p style=\"text-align: justify;\">Classical dendritic cells (cDCs) are rare sentinel cells specialized in the regulation of adaptive immunity. Modeling cDC development is crucial to study cDCs and harness their therapeutic potential. Here we address whether cDCs could differentiate in response to trophic cues delivered by mesenchymal components of the hematopoietic niche. We find that mesenchymal stromal cells engineered to express membrane-bound FLT3L and stem cell factor (SCF) together with CXCL12 induce the specification of human cDCs from CD34<sup>+<\/sup>\u00a0hematopoietic stem and progenitor cells (HSPCs). Engraftment of engineered mesenchymal stromal cells (eMSCs) together with CD34<sup>+<\/sup>\u00a0HSPCs creates an in vivo synthetic niche in the dermis of immunodeficient mice driving the differentiation of cDCs and CD123<sup>+<\/sup>AXL<sup>+<\/sup>CD327<sup>+<\/sup>\u00a0pre\/AS-DCs. cDC2s generated in vivo display higher levels of resemblance with human blood cDCs unattained by in vitro-generated subsets. Altogether, eMSCs provide a unique platform recapitulating the full spectrum of cDC subsets enabling their functional characterization in vivo.<\/p>\r\n<\/div>\r\n<p>[:]<\/p>","documents":null},"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Engineered niches support the development of human dendritic cells in humanized mice - CRI<\/title>\n<meta name=\"robots\" content=\"noindex, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Engineered niches support the development of human dendritic cells in humanized mice - CRI\" \/>\n<meta property=\"og:url\" content=\"https:\/\/cri1149.mlcom-dev.net\/publication\/engineered-niches-support-the-development-of-human-dendritic-cells-in-humanized-mice\/\" \/>\n<meta property=\"og:site_name\" content=\"CRI\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/CRI-Centre-de-recherche-sur-linflammation-100116118884153\" \/>\n<meta property=\"article:modified_time\" content=\"2025-09-11T14:47:58+00:00\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/cri1149.mlcom-dev.net\/publication\/engineered-niches-support-the-development-of-human-dendritic-cells-in-humanized-mice\/\",\"url\":\"https:\/\/cri1149.mlcom-dev.net\/publication\/engineered-niches-support-the-development-of-human-dendritic-cells-in-humanized-mice\/\",\"name\":\"Engineered niches support the development of human dendritic cells in humanized mice - 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