{"id":66502,"date":"2023-05-11T12:46:34","date_gmt":"2023-05-11T10:46:34","guid":{"rendered":"https:\/\/cri1149.mlcom-dev.net\/?post_type=publications&#038;p=66502"},"modified":"2025-09-11T16:47:57","modified_gmt":"2025-09-11T14:47:57","slug":"maldi-imaging-a-powerful-multiplex-approach-to-decipher-intratumoral-heterogeneity-combined-hepato-cholangiocarcinomas-as-proof-of-concept","status":"publish","type":"publication","link":"https:\/\/cri1149.mlcom-dev.net\/en\/publication\/maldi-imaging-a-powerful-multiplex-approach-to-decipher-intratumoral-heterogeneity-combined-hepato-cholangiocarcinomas-as-proof-of-concept\/","title":{"rendered":"MALDI Imaging, a Powerful Multiplex Approach to Decipher Intratumoral Heterogeneity: Combined Hepato-Cholangiocarcinomas as Proof of Concept"},"content":{"rendered":"","protected":false},"featured_media":0,"template":"","meta":{"_acf_changed":false},"class_list":["post-66502","publication","type-publication","status-publish","hentry"],"acf":{"numero_de_publication":"Cancers.","date_de_publication":"20230401","numero_doi":"","equipe":[121],"auteurs-liste":[{"texte_libre":true,"auteur-lien":null,"auteur-text":"Elia Gigante"},{"texte_libre":false,"auteur-lien":14852,"auteur-text":""},{"texte_libre":false,"auteur-lien":13019,"auteur-text":""},{"texte_libre":false,"auteur-lien":13096,"auteur-text":""},{"texte_libre":false,"auteur-lien":63210,"auteur-text":""},{"texte_libre":false,"auteur-lien":13135,"auteur-text":""}],"auteurs-manuel":"","liens_externes":null,"liens":[{"lien":"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC10093162\/"}],"paragraphe":"<p style=\"text-align: justify;\">Les travaux de <strong>th\u00e8se de Elia Gigante<\/strong> et de <strong>l'ing\u00e9nieur de la platefome iMAP H\u00e9l\u00e8ne Cazier <\/strong>de l'\u00e9quipe de <strong>Val\u00e9rie Paradis<\/strong> ont permis une caract\u00e9risation mol\u00e9culaire des h\u00e9patocholangiocarcinomes, un sous-type de tumeurs primitives malignes h\u00e9patiques de diagnostic difficile en raison de leur h\u00e9t\u00e9rog\u00e9n\u00e9it\u00e9 intratumorale importante. La comparaison des profils mol\u00e9culaires acquis par imagerie MALDI sur l\u2019ensemble des coupes tissulaires fix\u00e9es a identifi\u00e9 la pr\u00e9sence des deux composantes tumorales (carcinome h\u00e9patocellulaire et cholangiocarcinome) et a permis de mettre en \u00e9vidence des zones tumorales microscopiques intriqu\u00e9es non rep\u00e9r\u00e9es au microscope. Ce travail pr\u00e9sente une nouvelle application de l\u2019imagerie MALDI (imagerie <em>in silico<\/em>) offrant une approche d\u2019immunohistochimie multiplex virtuelle.<\/p>\r\n<p>Voici le type d'image et d'analyse obtenues gr\u00e2ce \u00e0 l'imagerie MALDI :<\/p>\r\n<p><img class=\" wp-image-66503 aligncenter\" src=\"https:\/\/cri1149.mlcom-dev.net\/wp-content\/uploads\/2023\/05\/Elia-Maldi-300x115.png\" alt=\"\" width=\"540\" height=\"207\" \/><\/p>\r\n<p style=\"text-align: center;\"><span style=\"font-size: 8pt;\"><em>Analyse de segmentation superpos\u00e9e \u00e0 l'immunohistochimie r\u00e9alis\u00e9e sur des tranches successives d'un h\u00e9patocholangiocarcinome. (A) Annotations pathologiques indiquant les zones de cholangiocarcinome (vert), carcinome h\u00e9patocellulaire (rouge), fibrose dans le cholangiocarcinome (bleu) * MVI. (B) Analyse de segmentation \u00e0 partir de l'imagerie MALDI identifiant 4 distincts. zones de fibrose li\u00e9e au cholangiocarcinome (bleu) ; HCC (rouge); iCCA (vert) ; et fibrose autour des nodules cirrhotiques (jaune).<\/em><\/span><\/p>","paragraphe_en":"<p style=\"text-align: justify;\">Combined hepato-cholangiocarcinomas (cHCC-CCA) belong to the spectrum of primary liver carcinomas, which include hepatocellular carcinomas (HCC) and intrahepatic cholangiocarcinomas (iCCA) at both ends of the spectrum. Mainly due to the high intratumor heterogeneity of cHCC-CCA, its diagnosis and pathological description remain challenging. Taking advantage of in situ non-targeted molecular mapping provided by MALDI (Matrix Assisted Laser Desorption Ionization) imaging, we sought to develop a multiscale and multiparametric morphological approach, integrating molecular and conventional pathological analysis. MALDI imaging was applied to five representative cases of resected cHCC-CCA. Principal component analysis and segmentations with MALDI imaging techniques identified areas related to either iCCA or HCC and also hidden tumor areas not visible microscopically. In addition, the overlap between MALDI segmentation and immunostaining provided a comprehensive description of cHCC-CCA tumor heterogeneity by identifying transitional and micro-metastatic areas. Moreover, a list of peptides derived from in silico digestion was obtained for each immunohistochemical marker and was matched within the peptide peak list acquired by MALDI. Comparison of immunostaining images with ions from in silico digestion revealed an accurate identification of iCCA and HCC areas. Our study provides further evidence on the performance of MALDI imaging in exploring intratumor heterogeneity and offering virtual multiplex immunostaining through a single acquisition.<\/p>\r\n<p>Here is the type of image and analysis obtained thanks to MALDI imaging:<\/p>\r\n<p><img class=\" wp-image-66503 aligncenter\" src=\"https:\/\/cri1149.mlcom-dev.net\/wp-content\/uploads\/2023\/05\/Elia-Maldi-300x115.png\" alt=\"\" width=\"503\" height=\"193\" \/><\/p>\r\n<p style=\"text-align: center;\"><span style=\"font-size: 8pt;\"><em>Segmentation analysis overlaid with immunohistochemistry performed on successive slices from a hepatocholangiocarcinoma. (<strong>A<\/strong>) Pathological annotations indicating areas of cholangiocarcinoma (green), hepatocellular carcinoma (red), fibrosis within cholangiocarcinoma (blue), * MVI. (<strong>B<\/strong>) Segmentation analysis from MALDI imaging identifying 4 distinct. areas cholangiocarcinoma-related fibrosis (blue); HCC (red); iCCA (green); and fibrosis around cirrhotic nodules (yellow).<\/em><\/span><\/p>\r\n<p>&nbsp;<\/p>\r\n<p>&nbsp;<\/p>","documents":null},"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>MALDI Imaging, a Powerful Multiplex Approach to Decipher Intratumoral Heterogeneity: Combined Hepato-Cholangiocarcinomas as Proof of Concept - CRI<\/title>\n<meta name=\"robots\" content=\"noindex, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"MALDI Imaging, a Powerful Multiplex Approach to Decipher Intratumoral Heterogeneity: Combined Hepato-Cholangiocarcinomas as Proof of Concept - CRI\" \/>\n<meta property=\"og:url\" content=\"https:\/\/cri1149.mlcom-dev.net\/publication\/maldi-imaging-a-powerful-multiplex-approach-to-decipher-intratumoral-heterogeneity-combined-hepato-cholangiocarcinomas-as-proof-of-concept\/\" \/>\n<meta property=\"og:site_name\" content=\"CRI\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/CRI-Centre-de-recherche-sur-linflammation-100116118884153\" \/>\n<meta property=\"article:modified_time\" content=\"2025-09-11T14:47:57+00:00\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/cri1149.mlcom-dev.net\/publication\/maldi-imaging-a-powerful-multiplex-approach-to-decipher-intratumoral-heterogeneity-combined-hepato-cholangiocarcinomas-as-proof-of-concept\/\",\"url\":\"https:\/\/cri1149.mlcom-dev.net\/publication\/maldi-imaging-a-powerful-multiplex-approach-to-decipher-intratumoral-heterogeneity-combined-hepato-cholangiocarcinomas-as-proof-of-concept\/\",\"name\":\"MALDI Imaging, a Powerful Multiplex Approach to Decipher Intratumoral Heterogeneity: Combined Hepato-Cholangiocarcinomas as Proof of Concept - CRI\",\"isPartOf\":{\"@id\":\"https:\/\/cri1149.mlcom-dev.net\/#website\"},\"datePublished\":\"2023-05-11T10:46:34+00:00\",\"dateModified\":\"2025-09-11T14:47:57+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/cri1149.mlcom-dev.net\/publication\/maldi-imaging-a-powerful-multiplex-approach-to-decipher-intratumoral-heterogeneity-combined-hepato-cholangiocarcinomas-as-proof-of-concept\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/cri1149.mlcom-dev.net\/publication\/maldi-imaging-a-powerful-multiplex-approach-to-decipher-intratumoral-heterogeneity-combined-hepato-cholangiocarcinomas-as-proof-of-concept\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/cri1149.mlcom-dev.net\/publication\/maldi-imaging-a-powerful-multiplex-approach-to-decipher-intratumoral-heterogeneity-combined-hepato-cholangiocarcinomas-as-proof-of-concept\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Accueil\",\"item\":\"https:\/\/cri1149.mlcom-dev.net\/en\/cri-home\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Publications\",\"item\":\"https:\/\/cri1149.mlcom-dev.net\/publications\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"MALDI Imaging, a Powerful Multiplex Approach to Decipher Intratumoral Heterogeneity: Combined Hepato-Cholangiocarcinomas as Proof of Concept\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/cri1149.mlcom-dev.net\/#website\",\"url\":\"https:\/\/cri1149.mlcom-dev.net\/\",\"name\":\"CRI - Centre de Recherche sur l'Inflammation\",\"description\":\"Centre de Recherche sur l&#039;Inflammation\",\"publisher\":{\"@id\":\"https:\/\/cri1149.mlcom-dev.net\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/cri1149.mlcom-dev.net\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"},{\"@type\":\"Organization\",\"@id\":\"https:\/\/cri1149.mlcom-dev.net\/#organization\",\"name\":\"CRI1149\",\"url\":\"https:\/\/cri1149.mlcom-dev.net\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/cri1149.mlcom-dev.net\/#\/schema\/logo\/image\/\",\"url\":\"https:\/\/cri1149.mlcom-dev.net\/wp-content\/uploads\/2014\/11\/8.4.2014-logo-CRI.jpg\",\"contentUrl\":\"https:\/\/cri1149.mlcom-dev.net\/wp-content\/uploads\/2014\/11\/8.4.2014-logo-CRI.jpg\",\"width\":215,\"height\":176,\"caption\":\"CRI1149\"},\"image\":{\"@id\":\"https:\/\/cri1149.mlcom-dev.net\/#\/schema\/logo\/image\/\"},\"sameAs\":[\"https:\/\/www.facebook.com\/CRI-Centre-de-recherche-sur-linflammation-100116118884153\",\"https:\/\/www.linkedin.com\/company\/cri-centre-de-recherche-sur-l-inflammation\/\"]}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"MALDI Imaging, a Powerful Multiplex Approach to Decipher Intratumoral Heterogeneity: Combined Hepato-Cholangiocarcinomas as Proof of Concept - CRI","robots":{"index":"noindex","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"og_locale":"en_US","og_type":"article","og_title":"MALDI Imaging, a Powerful Multiplex Approach to Decipher Intratumoral Heterogeneity: Combined Hepato-Cholangiocarcinomas as Proof of Concept - CRI","og_url":"https:\/\/cri1149.mlcom-dev.net\/publication\/maldi-imaging-a-powerful-multiplex-approach-to-decipher-intratumoral-heterogeneity-combined-hepato-cholangiocarcinomas-as-proof-of-concept\/","og_site_name":"CRI","article_publisher":"https:\/\/www.facebook.com\/CRI-Centre-de-recherche-sur-linflammation-100116118884153","article_modified_time":"2025-09-11T14:47:57+00:00","twitter_card":"summary_large_image","schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/cri1149.mlcom-dev.net\/publication\/maldi-imaging-a-powerful-multiplex-approach-to-decipher-intratumoral-heterogeneity-combined-hepato-cholangiocarcinomas-as-proof-of-concept\/","url":"https:\/\/cri1149.mlcom-dev.net\/publication\/maldi-imaging-a-powerful-multiplex-approach-to-decipher-intratumoral-heterogeneity-combined-hepato-cholangiocarcinomas-as-proof-of-concept\/","name":"MALDI Imaging, a Powerful Multiplex Approach to Decipher Intratumoral Heterogeneity: Combined Hepato-Cholangiocarcinomas as Proof of Concept - CRI","isPartOf":{"@id":"https:\/\/cri1149.mlcom-dev.net\/#website"},"datePublished":"2023-05-11T10:46:34+00:00","dateModified":"2025-09-11T14:47:57+00:00","breadcrumb":{"@id":"https:\/\/cri1149.mlcom-dev.net\/publication\/maldi-imaging-a-powerful-multiplex-approach-to-decipher-intratumoral-heterogeneity-combined-hepato-cholangiocarcinomas-as-proof-of-concept\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/cri1149.mlcom-dev.net\/publication\/maldi-imaging-a-powerful-multiplex-approach-to-decipher-intratumoral-heterogeneity-combined-hepato-cholangiocarcinomas-as-proof-of-concept\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/cri1149.mlcom-dev.net\/publication\/maldi-imaging-a-powerful-multiplex-approach-to-decipher-intratumoral-heterogeneity-combined-hepato-cholangiocarcinomas-as-proof-of-concept\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Accueil","item":"https:\/\/cri1149.mlcom-dev.net\/en\/cri-home\/"},{"@type":"ListItem","position":2,"name":"Publications","item":"https:\/\/cri1149.mlcom-dev.net\/publications\/"},{"@type":"ListItem","position":3,"name":"MALDI Imaging, a Powerful Multiplex Approach to Decipher Intratumoral Heterogeneity: Combined Hepato-Cholangiocarcinomas as Proof of Concept"}]},{"@type":"WebSite","@id":"https:\/\/cri1149.mlcom-dev.net\/#website","url":"https:\/\/cri1149.mlcom-dev.net\/","name":"CRI - Centre de Recherche sur l'Inflammation","description":"Centre de Recherche sur l&#039;Inflammation","publisher":{"@id":"https:\/\/cri1149.mlcom-dev.net\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/cri1149.mlcom-dev.net\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Organization","@id":"https:\/\/cri1149.mlcom-dev.net\/#organization","name":"CRI1149","url":"https:\/\/cri1149.mlcom-dev.net\/","logo":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/cri1149.mlcom-dev.net\/#\/schema\/logo\/image\/","url":"https:\/\/cri1149.mlcom-dev.net\/wp-content\/uploads\/2014\/11\/8.4.2014-logo-CRI.jpg","contentUrl":"https:\/\/cri1149.mlcom-dev.net\/wp-content\/uploads\/2014\/11\/8.4.2014-logo-CRI.jpg","width":215,"height":176,"caption":"CRI1149"},"image":{"@id":"https:\/\/cri1149.mlcom-dev.net\/#\/schema\/logo\/image\/"},"sameAs":["https:\/\/www.facebook.com\/CRI-Centre-de-recherche-sur-linflammation-100116118884153","https:\/\/www.linkedin.com\/company\/cri-centre-de-recherche-sur-l-inflammation\/"]}]}},"post_type":"publication","author_name":"","_links":{"self":[{"href":"https:\/\/cri1149.mlcom-dev.net\/en\/wp-json\/wp\/v2\/publication\/66502","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/cri1149.mlcom-dev.net\/en\/wp-json\/wp\/v2\/publication"}],"about":[{"href":"https:\/\/cri1149.mlcom-dev.net\/en\/wp-json\/wp\/v2\/types\/publication"}],"acf:term":[{"embeddable":true,"taxonomy":"equipes","href":"https:\/\/cri1149.mlcom-dev.net\/en\/wp-json\/wp\/v2\/equipes\/121"}],"wp:attachment":[{"href":"https:\/\/cri1149.mlcom-dev.net\/en\/wp-json\/wp\/v2\/media?parent=66502"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}